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微囊化重组CHO 细胞移植促进大鼠心肌梗死后血管新生的实验研究

《中国胸心血管外科临床杂志》[ISSN:1007-4848/CN:51-1492/R]

期数:
2008年第15卷第1期
页码:
38-43
栏目:
基础研究论著
出版日期:
2008-02-25

文章信息/Info

Title:
Transplantation of Microencapsulated Recombinanted Chinese Hamster Ovary Cells Promotes Angiogenes is in Postinfarction Myocardium in Rats
文章编号:
1007-4848 (2008) 01-0038-06
作者:
朱沈军1; 张浩1; 胡盛寿1; 王为2; 张虹1; 李君1; 魏英杰1
1. 中国医学科学院中国协和医科大学卫生部心血管疾病再生医学研究重点实验室     阜外心血管病医院外科, 北京100037; 2. 中国科学院大连化学物理研究所, 辽宁大连116023
Author(s):
ZHU Shen-jun1;  ZHANG Hao1;  HU Sheng -shou1;  WANG Wei2;  ZHANG Hong 1; LI Jun1;  WEI Y ing -j ie1.
1. Research Center for Card iovascular Regenerative Medicine, the Ministry of Health,Department of Cardiovascular S urgery , Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100037, P. R. China; 2. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning , P. R. China
关键词:
微囊;  　血管内皮生长因子;  　心肌梗死;  　细胞治疗;  　血管新生
Keywords:
M icrocapsule;  　Vascular endothelial grow th factor;  　Myocardial infarction;  　Cell therapy;  　
A ngiogenesis
分类号:
R542. 2+ 2; Q 813. 1+ 1
DOI:
-
文献标识码:
A
摘要:
摘要: 　目的　在大鼠心肌梗死部位植入微囊化重组中华仓鼠卵巢(chinese hamster ovary, CHO ) 细胞, 观察其分泌的血管内皮细胞生长因子(V EGF) 是否可以促进心肌梗死部位的血管新生, 改善心功能。　方法　通过质粒转染的方法构建可以分泌V EGF 的重组CHO 细胞系, 用微囊包裹, 观察微囊内细胞的生长及分泌情况。制备大鼠心肌梗死模型, 随机将48 只8 周龄SD 雄性大鼠分成微囊化细胞移植组(MC2CHO 组)、单纯细胞移植组(CHO 组)、空微囊移植组(MC 组) 和无血清培养基移植组(对照组) , 每组12 只。移植3 周后检测心功能改善情况, 病理切片观察微囊结构及囊内细胞存活情况, 注射部位微血管计数比较促血管新生效果。　结果　体外检测可见重组CHO 细胞在微囊生长迅速, 第8 d 时培养上清中V EGF 达3 852 pgöm l; 移植3 周后MC2CHO 组左心室大小和心功能明显好于其它3 组, 差异有统计学意义(P < 0. 05) ; 局部微血管密度MC2CHO 组较CHO 组、MC 组和对照组明显增加(22. 3±3. 1 vs. 15. 6±2.8, 11. 4±2. 5 和13. 2±2. 7 个ö每高倍视野, P < 0. 05) ; 组织学检查可见微囊结构完整, 内有存活且具有分泌功能的CHO 细胞。　结论　微囊化重组CHO 细胞移植可促进大鼠心肌梗死后血管新生, 改善心功能。
Abstract:
Abstract: 　Objective　To transplant the microencapsulated recombinanted Chinese hamster ovary (CHO ) cells into the infracted myocardium of rodent animals and investigate whether vascular endothelial growth factor (VEGF) secreted by the implanted CHO cells could augment angiogenesis and improve cardiac function. 　Methods　The cDNA of VEGF was transferred into CHO cells with plasmid stable transfection. After microencapsulation, the cell growth in microcapsules and the VEGF level in the culture supernatant were evaluated. Two weeks after myocardial infarction, the microencapsulated CHO cells (MC-CHO group ) were implanted into the border of infracted myocardium, as well as similar amount of CHO cells (CHO group ) , blank microcapsule (MC group ) and non-serum culture medium (control group ) as controls, 12 rats per group. The cardiac function improvement was evaluated 3 weeks after transplantation, while the survival status of implanted CHO cells, in situ secretion of VEGF and capillary density were assayed by histology. 　Results　CHO cells could grow and proliferate after microencapsulation. The secretion of VEGF was detectable in culture media supernatant, with the highest concentration of 3 852 pg/m l at day 8. As compared to the other three groups, the left ventricular dimension and cardiac function were significantly improved in MC-CHO group 3 weeks after transplantation. The capillary density of MC-CHO group were increased significantly than those of CHO group, MC group and control group (22. 3±3. 1 vs. 15. 6±2. 8, 11. 4±2. 5, 13. 2±2. 7 vessels per 0.13 mm2, P < 0.05). The implanted microcapsule maintained its original shape and protected theCHO cells in it. 　Conclusion 　M icroencapsulaed recombinanted CHO cells transplantation might be a promising app roach to augment angiogenesis and improve the cardiac function in infarction myocardium.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2008-06-11