平滑肌细胞移植对心肌梗死后早期心肌基质金属蛋白酶2、9和抑制因子3含量的影响()
《中国胸心血管外科临床杂志》[ISSN:1007-4848/CN:51-1492/R]
- 期数:
- 2010年第17卷第3期
- 页码:
- 219-224
- 栏目:
- 基础研究论著
- 出版日期:
- 2010-06-25
文章信息/Info
- Title:
- The Effect of Smooth Muscle Cell Transplantation on MyocardialMMP2, MMP9 and TIMP3 Content One Week after Myocardial Infarction
- 文章编号:
- 1007-4848（2010）03-0219-06
- 作者:
- 刘开宇 田海 刘伟 等
- 1哈尔滨医科大学附属第二医院 心外科 教育部黑龙江省部共建心肌缺血机理与诊疗技术重点实验室，哈尔滨 150086； 2加拿大多伦多大学多伦多总医院 心血管外科，M5G2C4加拿大
- Author(s):
- LIU Kai-yu; TIAN Hai; LIU Wei; et al .
- 1. Department of Cardiovascular Surgery, the Second Affiliated Hospital, Harbin Medical University, Key Laboratories of Education Ministry for Myocardial Ischemia Mechanism and Treatment, Harbin 150086, P. R. China; 2. Department of Cardiovascular Surgery, Toronto General Hospital, University of Toronto, M5G2C4 Canada
- 关键词:
- ; 平滑肌细胞移植; 心肌重构; 基质金属蛋白酶抑制因子3; 基质金属蛋白酶2; 基质金属蛋白酶9
- Keywords:
- Smooth muscle cell transplantation; Myocardial remodeling; Tissue inhibitor of metalloprotease-3; Matrix metalloproteinase-2; Matrix metalloproteinase-9
- 分类号:
- R541.4; Q813.3
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的 评价平滑肌细胞移植对心肌梗死后早期心肌间质重构的影响。 方法 选用48只雌性Wistar大鼠，采用随机数字表法分成对照组（n=24）和平滑肌细胞移植组（n=24），经左冠状动脉远端结扎后建立心肌梗死动物模型，立即对梗死区边缘行室壁注射含有1×106个平滑肌细胞或不含细胞的磷酸盐缓冲液(PBS)0.5 ml。在移植后1周，通过逆转录聚合酶链反应(RT-PCR)和免疫杂交观察大鼠心肌内基质金属蛋白酶2、9（MMP-2、MMP-9）和基质金属蛋白酶抑制因子3（TIMP-3）的信使核糖核酸（mRNA）和蛋白变化。 结果 植入的平滑肌细胞能够存活；平滑肌细胞移植组大鼠缺血区TIMP-3 mRNA（1.06±0.22 vs. 0.81±0.19，t=－2.358，P=0.033）及其蛋白含量（333±0.53 vs. 1.63±0.47，t=－6.802，P<0.001）明显高于对照组；平滑肌细胞移植组大鼠缺血区MMP2、MMP-9 mRNA（0.49±0.12 vs. 1.16±0.18，t=8.453，P<0.001；0.45±0.12 vs. 0.80±0.11，t=5.884，P<0.001）及其蛋白含量（3.98±1.08 vs. 6.05±0.91，t=4.139，P=0.001；0.39±0.14 vs. 0.57±0.17，t=2.409，P=0.031）明显低于对照组。 结论 移植的平滑肌细胞可在心肌梗死区及其周围存活，并且增加梗死后心肌中TIMP3 mRNA和蛋白的含量，降低MMP2、MMP9 mRNA和蛋白含量，抑制心肌不良重构。
- Abstract:
- Objective To evaluate the effect of smooth muscle cell transplantation on myocardial interstitial reconstruction shortly after myocardial infarction. Methods A total of 48 female Wister rats were randomly divided into two groups with the random number table, the control group (n=24) and the smooth muscle cell transplantation group (n=24). The left coronary artery was ligated to set up the myocardial infarction animal model. An amount of 05 ml phosphate buffered saline(PBS) containing 1×106 smooth muscle cells or 0.5 ml PBS without cells was injected into the injured myocardium immediately. By immunoblot and reverse transcriptionolymerase china reaction (RT-PCR), we observed the amount of protein and mRNA of matrix metalloproteinase2(MMP-2), matrix metalloproteinase-9(MMP-9) and tissue inhibitor of metalloprotease-3 (TIMP-3) in the myocardium of the rats. Results The transplanted smooth muscle cells survived well. Compared with the control group, myocardial TIMP3 mRNA (1.06±0.22 vs. 0.81±0.19, t=－2.358, P=0.033) and protein content (3.33±0.53 vs. 1.63±0.47, t=－6.802, P<0.001) were significantly increased in the transplantation group. Myocardial MMP-2, MMP-9 mRNA (0.49±0.12 vs. 1.16±0.18, t=8.453, P<0.001; 0.45±0.12 vs. 0.80±0.11, t=5.884, P<0.001) and protein content (3.98±1.08 vs. 6.05±0.91, t=4.139, P=0.001; 0.39±0.14 vs. 0.57±0.17, t=2.409, P=0.031) [CM(1585mm]were significantly reduced in the transplantation group compared with the control group. Conclusion transplanted smooth muscle cells can survive well in the infarction myocardium and can increase the amount of myocardial TIMP-3 mRNA and protein content and reduce myocardial MMP-2, MMP-9 mRNA and protein content, which is an effective way to prevent harmful cardiac remodeling.
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更新日期/Last Update: 2010-07-06