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Title:: Fluoro Jadec Staining and Brain Injury after Deep Hypothermia Circulatory Arrest

Author(s):: WANG Ren; MA Wei-guo; GAO Guo-dong; et al .; Vascular Center, Fu Wai Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100037, P.R. China

摘要:: 目的评估Fluoro Jade-c（FJC）染色对深低温停循环术后脑损伤的检测效果。方法建立能模拟临床深低温停循环术后脑损伤的动物模型，将6头中华小型猪行深低温停循环，并保证所有动物存活，对动物脑组织进行病理学检测，动物脑组织灌注固定后行FJC 染色，分别取染色阳性和阴性部位，行脑组织苏木精伊红（Hematoxylineosin，HE）染色、尼氏（Nissl）染色、原位凋亡（Terminal deoxynucleotidyl Transferase BiotindUTP Nick End Labeling，TUNEL）检测；检测结果对照：FJC染色结果与TUNEL检测、HE染色、Nissl染色结果相对照，验证FJC染色对深低温停循环术后脑损伤检测的准确性和可靠性。结果实验动物术后FJC 染色发现有阳性病灶。FJC染色与TUNEL(Kappa值＝0.526，P<0.01)，Nissl染色(Kappa值＝0.555，P<0.01)，HE染色（Kappa值＝0491，P<0.01）对比，结果均具有一致性；FJC染色图象更清晰，更易于辨认变性神经元细胞。结论 FJC染色是一种可靠和更方便的检测深低温停循环后脑损伤的方法。

Abstract:: Objective To assess the results of Fluoro Jade-c (FJC) staining in brain injury after deep hypothermia circulatory arrest (DHCA). Methods First, animal model of DHCA were established. We performed DHCA on six Chinese experimental minipigs and made sure all the pigs were alive after operation. Second, pathological examination was carried out on the brain tissues of these animals. After FJC staining, we respectively took out the positive and negative tissueparts and performed Hematoxylineosin (HE) staining, Nissl staining and Terminal deoxynucleotidyl Transferase BiotindUTP Nick End Labeling (TUNEL). Finally, the results of FJC was compared with TUNEL, Nissl staining, HE staining, to verif
y the accuracy and reliability of FJC in assessing brain injury after DHCA. Results Postoperative FJC staining discovered positive disease focuses on the experimental pigs. The comparative results of FJC were consistent with TUNEL (Kappa=0.526, P<0.01), Nissl staining (Kappa=0.555, P<0.01) and HE staining (Kappa=0.491, P<0.01). However, FJC staining image was much clearer and easier in identifying brain injury. Conclusion FJC is a reliable and convenient method to assess brain injury after DHCA.